Regulatory

Part:BBa_K201008:Design

Designed by: francesca ceroni   Group: iGEM09_Bologna   (2009-10-12)
Revision as of 14:07, 21 October 2009 by Marilisa (Talk | contribs) (→‎Design Notes)

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LacI repressed BBa_J23100 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 625
    Illegal AgeI site found at 737
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed with the same structure of the Berkley constitutive promoters. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. As a second passage, the obtained O2-RFP part was digested with XbaI and PstI and ligated downstream of the BBa_J23118 promoter digested with SpeI and PstI.This was done to allow an easier cloning of the operator and promoter sequences that are short in length (about 20 bp and 35 bp, respectively).

Source

All subparts come from the Registry of Standard Parts

References