Regulatory

Part:BBa_K201004:Design

Designed by: francesca ceroni   Group: iGEM09_Bologna   (2009-10-12)
Revision as of 13:59, 21 October 2009 by Marilisa (Talk | contribs) (→‎Design Notes)

Lac O2 natural operator with RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 582
    Illegal AgeI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed with the same structure of the Berkley constitutive promoters. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp).

Source

All subparts come from the Registry of Standard Parts

References