Plasmid

Part:BBa_K5143025

Designed by: Perrel Aymeric   Group: iGEM24_UnivLyon1-INSALyon   (2024-09-18)
Revision as of 20:58, 18 September 2024 by Registry (Talk | contribs)

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Plasmid D

This part was designed to be used in Saccharomyces cerevisiae. By digesting this part with XhoI, the linearize fragment could be transformed in the yeast in order to recombinate with the Ura locus in S. cerevisiae BY4741 strain. Then, the yeast will express the alphafactor-fwYellow-CBD gene and the alphafactor-MaSp1-CBD gene. In our project, these two proteins will be secreted by the yeast and will bind to the cellulose (thanks to the fused CBD) in order to functionalize the cellulose.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3567
    Illegal EcoRI site found at 1361
    Illegal EcoRI site found at 7006
    Illegal EcoRI site found at 7533
    Illegal PstI site found at 2789
    Illegal PstI site found at 3092
    Illegal PstI site found at 3185
    Illegal PstI site found at 3191
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1361
    Illegal EcoRI site found at 7006
    Illegal EcoRI site found at 7533
    Illegal SpeI site found at 3568
    Illegal PstI site found at 2789
    Illegal PstI site found at 3092
    Illegal PstI site found at 3185
    Illegal PstI site found at 3191
    Illegal PstI site found at 3582
    Illegal NotI site found at 3575
    Illegal NotI site found at 7539
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1361
    Illegal EcoRI site found at 7006
    Illegal EcoRI site found at 7533
    Illegal BamHI site found at 1906
    Illegal BamHI site found at 2620
    Illegal XhoI site found at 5171
    Illegal XhoI site found at 7012
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3568
    Illegal EcoRI site found at 1361
    Illegal EcoRI site found at 7006
    Illegal EcoRI site found at 7533
    Illegal PstI site found at 2789
    Illegal PstI site found at 3092
    Illegal PstI site found at 3185
    Illegal PstI site found at 3191
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 7533
    Illegal EcoRI site found at 1361
    Illegal EcoRI site found at 7006
    Illegal SpeI site found at 3568
    Illegal PstI site found at 2789
    Illegal PstI site found at 3092
    Illegal PstI site found at 3185
    Illegal PstI site found at 3191
    Illegal PstI site found at 3582
    Illegal NgoMIV site found at 1876
    Illegal AgeI site found at 1915
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4280


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