Composite

Part:BBa_K5043011:Design

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-09-14)
Revision as of 12:46, 18 September 2024 by Richard-meuten (Talk | contribs) (→‎Source)

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amilGFP transcriptional unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 161
    Illegal AgeI site found at 622
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

amilGFP was taken from iGEM 2024 distribution kit. RBS and restriction sides for BsaI were added using overhang-PCR to enable GoldenGate assembly.


Source

Derived from Acropora millepora. [9] Protein coding sequence was taken from iGEM 2024 distribution kit, regulatory elements were ordered from IDT.

References