Coding

Part:BBa_K5492203

Designed by: Norbert Nyeste, Sandor Ban, Vojtech Vasut, Sara Simonka   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-15)
Revision as of 20:16, 17 September 2024 by Noriii (Talk | contribs)


Optimised DAO - pETDUET-1

Optimised coding sequence of rat Diamine Oxidase appropiated for insertion into pETDUET-1.

Usage and Biology

Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine, and the resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal PstI site found at 190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal BglII site found at 2392
    Illegal BamHI site found at 123
    Illegal BamHI site found at 2067
    Illegal BamHI site found at 2321
    Illegal XhoI site found at 937
    Illegal XhoI site found at 2441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
    Illegal NgoMIV site found at 2411
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None