Regulatory

Part:BBa_K216005:Experience

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-09-25)
Revision as of 13:45, 21 October 2009 by Evangelineoh (Talk | contribs) (Applications of BBa_K216005)

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Applications of BBa_K216005

Edinburgh iGEM team, October 2009: To test this part, it was combined with lacZ minigene J33202 for Miller assays (giving part BBa_K216009), and also with GFP for analysis using the promoter characterization kit of Jason Kelly. Results from overnight induction with Miller assay indicated stronger induction by nitrite than nitrate, but a time course of induction using the GFP construct indicated that nitrate gave stronger induction in the first few hours.

Results from Miller's Assay
PyeaR-miller.png <p><p> Results from GFP promoter characterization kit.
Pyear characterization.jpg

User Reviews

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