Composite

Part:BBa_K5398620:Design

Designed by: ziyi guo   Group: iGEM24_NAU-CHINA   (2024-09-17)
Revision as of 07:19, 17 September 2024 by Registry (Talk | contribs)

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Use LicV fusion protein to improve the leakage of the BBa_K3739064 system and enable its function as


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
    Illegal AgeI site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The improvement of NAU-CHINA this year is to lower the leakage level of the BBa_K3739064. Aiming to control the leakage issue, we insert the RAT terminator sequence into the regions between the pBlind promoter sequence and superfolder(sfGFP) reporter gene sequence. Besides, the expression level of LicV is high enough to ensure that the sfGFP can transcript normally due to the strong promoter J23104.


Source

Our part is improved from BBa_K3739064 system designed by iGEM21_XMU-China

References