Composite

Part:BBa_K5317012

Designed by: Jan Gelhoet   Group: iGEM24_Hannover   (2024-09-16)
Revision as of 09:56, 16 September 2024 by Annaseidler (Talk | contribs)


CMV-MTF1-mRuby2

Usage and Biology

The Metal Regulatory Transcription Factor 1 (MTF-1) is a metal ion-sensing transcription factor, regulating primarily zinc, cadmium and copper homeostasis and detoxification (Tavera-Montañez et al. 2019, Wimmer et al. 2005). Activation of MTF-1 due to increasing levels of heavy metals in the cytoplasm results in its translocation into the nucleus and binding via its zinc finger domains to MREs, specifically consensus TGCRCNC in promoter regions of the DNA. Thereby MTF-1 regulates expression of metallothioneins, metal transporters, and antioxidant genes as protection against metal toxicity and oxidative stress (Tavera-Montañez et al. 2019). Additional stimuli of MTF-1 nucleus import are stress signals such as heat shock, H2O2, low extracellular pH (Saydam et al. 2001).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1785
    Illegal PstI site found at 1030
    Illegal PstI site found at 1362
    Illegal PstI site found at 1819
    Illegal PstI site found at 1886
    Illegal PstI site found at 1924
    Illegal PstI site found at 2341
    Illegal PstI site found at 2671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1785
    Illegal PstI site found at 1030
    Illegal PstI site found at 1362
    Illegal PstI site found at 1819
    Illegal PstI site found at 1886
    Illegal PstI site found at 1924
    Illegal PstI site found at 2341
    Illegal PstI site found at 2671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1785
    Illegal BamHI site found at 1220
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1785
    Illegal PstI site found at 1030
    Illegal PstI site found at 1362
    Illegal PstI site found at 1819
    Illegal PstI site found at 1886
    Illegal PstI site found at 1924
    Illegal PstI site found at 2341
    Illegal PstI site found at 2671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1785
    Illegal PstI site found at 1030
    Illegal PstI site found at 1362
    Illegal PstI site found at 1819
    Illegal PstI site found at 1886
    Illegal PstI site found at 1924
    Illegal PstI site found at 2341
    Illegal PstI site found at 2671
    Illegal NgoMIV site found at 2599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2710

Cloning

Theoretical Part Design

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Categories
Parameters
None