Part:BBa_K5317013

PknB

PknB is a eukaryote-like serine/threonine kinase in Staphylococcus aureus that plays an important role in the bacterial response to antibiotics, particularly beta-lactams, via its PASTA domain (Stehle et al.2012). PknB is a membrane-localized protein consisting of an N-terminal cytosolic kinase domain, a central transmembrane segment and three C-terminal extracellular PASTA domains. The PASTA (penicillin-binding protein and serine/threonine kinase-associated) domain plays a critical role in the recognition and binding of beta-lactam antibiotics (Stehle et al.2012). Upon binding these compounds, the PASTA domain initiates a signaling cascade by inducing autophosphorylation of the N-terminal kinase domain. This activation leads to the initiation of downstream signaling pathways (Cheung et al.2010). In S. aureus, this mechanism is critical for early detection of antibiotics and helps the bacteria adapt to antibiotic stress (Sauer et al. 2018).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1213
  Illegal SpeI site found at 1876
  Illegal PstI site found at 638
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1876
  Illegal PstI site found at 638
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 888
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1213
  Illegal SpeI site found at 1876
  Illegal PstI site found at 638
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1213
  Illegal SpeI site found at 1876
  Illegal PstI site found at 638
  
• 1000
  COMPATIBLE WITH RFC[1000]