Coding

Part:BBa_K5492203

Designed by: Norbert Nyeste, Sandor Ban, Vojtech Vasut, Sara Simonka   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-15)
Revision as of 11:34, 15 September 2024 by Registry (Talk | contribs)

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Optimised DAO - pETDUET-1

Optimised coding sequence of rat Diamine Oxidase appropiated for insertion into pT7CFE1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal PstI site found at 190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal BglII site found at 2392
    Illegal BamHI site found at 123
    Illegal BamHI site found at 2067
    Illegal BamHI site found at 2321
    Illegal XhoI site found at 937
    Illegal XhoI site found at 2441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
    Illegal NgoMIV site found at 2411
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None