Composite
Part:BBa_K5043011:Design
Designed by: Richard Meuten Group: iGEM24_TUDarmstadt (2024-09-14)
amilGFP transcriptional unit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 161
Illegal AgeI site found at 622 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
amilGFP was taken from iGEM 2024 distribution kit. RBS and restriction sides for BsaI were added using overhang-PCR to enable GoldenGate assembly.
Source
Acropora millepora. Protein coding sequence was taken from iGEM 2024 distribution kit, regulatory elements were ordered from IDT.