Part:BBa_K5226039

cysNC

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 176
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 667
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 532
  Illegal SapI.rc site found at 600
  

Introduction

The existing methods for large-scale production of P34HB primarily rely on microbial fermentation. A key limiting factor in this process is the molar ratio of 4HB. Increasing the 4HB molar ratio can lead to a decrease in the melting temperature and apparent fusion heat of the copolymer, as well as an improvement in the polymer's deformation resistance. Therefore, enhancing the molar ratio of 4HB is crucial for the modification of P34HB.

Usage and Biology

cysNC is multifunctional fusion protein.The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the synthesis of activated sulfate and the synthesis of activated sulfate.In addition, it can forms the ATP sulfurylase (ATPS) with CysD to catalyze the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway.