Part:BBa_K5083000

GLP-1

Glucagon-like peptide-1 (GLP-1) is a hormone primarily produced by intestinal L cells and belongs to the incretin family. GLP-1 functions include promoting insulin secretion, reducing glucagon secretion, inhibiting gastric emptying, and decreasing gastric acid secretion. GLP-1 can be used to treat type 2 diabetes and obesity. It is one of the incretin hormones, derived from the cleavage of proglucagon. Proglucagon is a peptide hormone composed of 160 amino acids and is found in intestinal L cells or the brain. After being cleaved by prohormone convertase 1 (PC1), it is split into two different forms of GLP-1: GLP-1 (7–36) amide, a 30-amino acid amidated form, and GLP-1 (7–37), a 31-amino acid form extended by glycine. The majority of biologically active GLP-1 in the human body exists as GLP-1 (7–36) amide. We demonstrated through ELISA and Western blotting that GLP-1 can be expressed in Escherichia coli BL21 and Nissle 1917.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]