Regulatory

Part:BBa_K5317006

Designed by: Jan Gelhoet   Group: iGEM24_Hannover   (2024-09-13)
Revision as of 09:14, 13 September 2024 by Registry (Talk | contribs)

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MREdada promoter

The MRE-sites containing promoter enables the metal-dependent expression of a downstream positioned reporter gene via the metal ion-dependent transcription factor MTF-1 for cell-based metal detection.

To combine the observations made by Searle and colleagues and Wang and colleagues in 1985 and 2004, respectively, regarding the metal inducibility of a promoter with two MREa sites and a high affinity between MREd and MTF-1, we designed a synthetic promoter with two MREa and two MREd sites that alternate. The aim is possibly increasing the sensitivity and efficiency of the metal-dependent promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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