RNA
Part:BBa_K5036011
Designed by: Emad hamdy Matter Group: iGEM24_AFCM-Egypt (2024-09-12)
Nanog gRNA2 of dCas9
Part Description
It is guide RNA which is a crucial component of the CRISPR-Cas9 gene editing system as it act as A guide the dCas9 to its target. It consist of two main parts: first Spacer Sequence: which is a short, user-defined sequence that complements the target DNA sequence you want to edit. Second Scaffold Sequence which is pre-designed RNA structure allows gRNA to bind to the Cas9 protein and facilitate its interaction with the target DNA
Usage
after the fusion of the two parts of dCas9 it will guide the(dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog enhancer of YAP for interaction and initiation of transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |