Coding

Part:BBa_K5036007

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-12)
Revision as of 08:27, 12 September 2024 by Registry (Talk | contribs)

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N-TEV

This is known as the tobacco etch virus which is very selective for cleaving proteins at particular amino acid sequences and has been modified to have better qualities like greater heat stability, decreased autolysis (self-cleavage) and also allows researchers to precisely cleave the tag off, leaving behind the receptor in its unmodified form. In our model, TEV was divided into N-terminal and C-terminal fragments. So the N-terminal fragment was grafted onto dCAS9(N) to generate TEV(N)-dCas9(N).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316


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Categories
Parameters
None