Part:BBa_K191003

Promoter Lac I - RBS - LovTAP - Term

Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.

For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] on team EPF-Lausanne wiki.

Usage and Biology

In order to be able to test the efficiency of the fused protein LovTAP, its expression is induced by IPTG ( thanks to the LacI promoter). Two other parts were also constructed as the read out systems of LovTAP : BBa_K191004 and BBa_K191005

All three parts together gives the following system, where LovTAP activities can be measured through RFP fluorescence intensity

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 370
  Illegal PstI site found at 627
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 370
  Illegal PstI site found at 627
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 370
  Illegal PstI site found at 627
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 370
  Illegal PstI site found at 627
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 764
  

Functional Parameters

input_s	IPTG
positive_regulators	-NA-
protein_out	LovTAP