Part:BBa_K5184021
rCtx-4
"Ctx-4 is an insecticidal peptide obtained from the ctenid spider Phoneutria depilata. It is consists of 53 amino acids, including 10 Cys residues that form 5 disulfide bonds. It's a venom peptide used by P. depilata to incapacitate their prey. Based on its original amino acid sequence, a synthetic gene is assembled through the use of overlapping PCR. The new gene is thus called recombinant Ctx-4, also abbreviated as rCtx-4. The sequence of it is annotated in the transcriptome as a sodium channel neurotoxin. However, during in vivo experiments, rCtx-4 is proven to only establish paralyzing effects on certain invertabrates such as crickets and mites. Meanwhile, mammals including mice were not affected by its toxicity. In our context, we attempted to construct the rCtx-4 sequence onto the vector pQE30 and transform it into E.coli to express the venom peptide. The venom peptide obtained is purified and injected into Tetrachynus urticae to establish paralyzing effects."
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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