Part:BBa_K5101005
pET29(a)-pJ23119-TIpA39-T-pTIpA-RBS-PhiX174E-T7
To enable the engineered bacteria to suicide when released into the environment (generally above 39 degrees Celsius) during fecal disposal, we chose a temperature-responsive protein TlpA39(BBa_K2572000) and its corresponding promoter pTlpA(BBa_K2500003) as regulatory elements to induce the expression of the lysis protein PhiX174E. TlpA39 is derived from Salmonella enterica. It is a temperature-sensitive protein that can bind to pTlpA. At temperatures above 39 degrees Celsius, the TlpA39 protein will remain in a monomeric form, thereby losing the ability to bind to the promoter pTlpA. If the temperature drops below 39 degrees Celsius, the TlpA39 protein will form a dimer; the dimer can inhibit the pTlpA and prevent the recruitment of RNA polymerase, thereby suppressing downstream gene expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1205
Illegal NotI site found at 572 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 857
Illegal XhoI site found at 1632 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1160
- 1000COMPATIBLE WITH RFC[1000]
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