Composite

Part:BBa_K180010

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-21)
Revision as of 11:29, 21 October 2009 by Registry (Talk | contribs)

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Rock-paper-scissors - Rock primary plasmid

Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.

Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 893
    Illegal NheI site found at 916
    Illegal PstI site found at 2760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 622
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2760
    Illegal AgeI site found at 3365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 147


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controltrp, lac
directionforward
emission610 nm
emit511
excitation587 nm
excite587
lum
n/aDsRed derivative
proteinmCherry