Composite
Part:BBa_K180010
Designed by: Richard Wilson, Stephanie Chambers Group: iGEM09_Southampton (2009-10-21)
Rock-paper-scissors - Rock primary plasmid
Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.
Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2760
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 893
Illegal NheI site found at 916
Illegal PstI site found at 2760 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 622
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2760
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2760
Illegal AgeI site found at 3365 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 147
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Categories
Parameters
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
control | trp, lac |
direction | forward |
emission | 610 nm |
emit | 511 |
excitation | 587 nm |
excite | 587 |
lum | |
n/a | DsRed derivative |
protein | mCherry |