Plasmid

Part:BBa_K5101001

Designed by: Ziqi Mi   Group: iGEM24_ZQT-Nanjing   (2024-09-10)
Revision as of 11:26, 10 September 2024 by Registry (Talk | contribs)

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Plasmid pET29a-pJ23119-RBS-AMP-T7

Plasmid pET29a-pJ23119-RBS-AMP-T7 is designed for robust expression of a novel antimicrobial peptide (AMP) in Escherichia coli BL21(DE3). This plasmid incorporates the strong constitutive promoter J23119 to drive the expression of AMP, aimed at providing a high-level output suitable for therapeutic applications. The RBS enhances the initiation of translation, ensuring efficient protein synthesis. The T7 terminator ensures proper transcriptional termination. This part has been constructed to study the antimicrobial effects of the peptide in vitro, with potential applications in treating bacterial infections in animal models.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 263
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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