Coding

Part:BBa_K180009:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-16)
Revision as of 11:09, 21 October 2009 by Rich (Talk | contribs) (→‎Design Notes)

mBanana (rights owned by Clontech)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
    Illegal NgoMIV site found at 598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 541


Design Notes

PstI site within the mCherry sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.

N.B. This part has not been submitted to the registry, as the rights to mCherry are commercially owned by Clontech. Academic researchers are welcome to use this part, subject to a material transfer agreement from the supplying university, providing that the DNA will not be used for commercial profit or gain. The sequence provided on this registry page has been provided with express permission from Clontech.

Source

pmBanana vector, Clontech

References

Shaner et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein. Nat Biotechnol (2004) vol. 22 (12) pp. 1567-1572