Part:BBa_K5226063

Mmp1 Vib C1M-porin58 Vib C3M

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE.

For the first method, based on previous studies obtained from literature research,[1][2][3][4]we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.

As a second approach, based on the homology between Vibrio natriegens and Halomonas TD [5], we chose to import the C1, C2, and C3 modules from Vibrio natriegens.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1199
  Illegal EcoRI site found at 3904
  Illegal EcoRI site found at 5277
  Illegal PstI site found at 4846
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1199
  Illegal EcoRI site found at 3904
  Illegal EcoRI site found at 5277
  Illegal NheI site found at 2161
  Illegal NheI site found at 4200
  Illegal PstI site found at 4846
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1199
  Illegal EcoRI site found at 3904
  Illegal EcoRI site found at 5277
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1199
  Illegal EcoRI site found at 3904
  Illegal EcoRI site found at 5277
  Illegal PstI site found at 4846
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1199
  Illegal EcoRI site found at 3904
  Illegal EcoRI site found at 5277
  Illegal PstI site found at 4846
  Illegal NgoMIV site found at 465
  
• 1000
  COMPATIBLE WITH RFC[1000]