Part:BBa_K5049003

Xylanase

Xylanases are enzymes that degrade xylan, a major component of plant cell walls, into simpler sugars. As feed additives, they play a crucial role in breaking down complex polysaccharides in animal diets, particularly for non-ruminants like poultry and swine. This enzymatic action enhances nutrient availability and digestion, leading to improved feed efficiency, growth performance, and overall health of the animals. Moreover, the supplementation of xylanase in animal feed can significantly reduce feed costs and environmental impact by increasing nutrient absorption and decreasing nutrient excretion.

We chose the xylanase gene from Streptomyces thermovulgaris for yeast surface display due to its exceptional properties. Researches on Streptomyces thermovulgaris have shown that its crude xylanase can hydrolyze KOH-treated corncob and produce bioactive xylooligosaccharides (XOs). The purified enzyme is highly stable across a broad pH range (4.0–11.5) and thermostable between 50–70°C, with maximum activity at 65°C and a half-life of 90 minutes at 70°C. Additionally, the enzyme’s products act as prebiotics that promote the growth of probiotic Lactobacillus plantarum, making it an ideal candidate for improving animal feed due to its stability, efficiency, and probiotic support.

REFERENCE

• Chakdar H, Kumar M, Pandiyan K, Singh A, Nanjappan K, Kashyap PL, Srivastava AK. Bacterial xylanases: biology to biotechnology. 3 Biotech. 2016 Dec;6(2):150. doi: 10.1007/s13205-016-0457-z. Epub 2016 Jun 30. PMID: 28330222; PMCID: PMC4929084.
• Ramatsui L, Sithole T, Mzimkulu-Ncoyi NH, Malgas S, Pletschke BI. The use of xylanases as additives to feeds: A mini-review of their effect on feed digestion and growth performance of monogastric animals. Microbial Bioprocesses. 2023 Jan 1:83-105.
• Patent: WO2020009964A1
• Patent: GB2585029A
• Boonchuay P, Techapun C, Seesuriyachan P, Chaiyaso T. Production of xylooligosaccharides from corncob using a crude thermostable endo-xylanase from Streptomyces thermovulgaris TISTR1948 and prebiotic properties. Food Science and Biotechnology. 2014 Oct;23:1515-23.
• Boonchuay P, Takenaka S, Kuntiya A, Techapun C, Leksawasdi N, Seesuriyachan P, Chaiyaso T. Purification, characterization, and molecular cloning of the xylanase from Streptomyces thermovulgaris TISTR1948 and its application to xylooligosaccharide production. Journal of Molecular Catalysis B: Enzymatic. 2016 Jul 1;129:61-8.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 529
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 844
  
• 1000
  COMPATIBLE WITH RFC[1000]