Coding
Part:BBa_K180008
Designed by: Richard Wilson, Stephanie Chambers Group: iGEM09_Southampton (2009-10-16)
mCherry (rights owned by Clontech)
mCherry fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through specially designed primers.
N.B. This part has not been submitted to the registry, as the rights to mCherry are commercially owned by Clontech. Academic researchers are welcome to use this part, subject to a material transfer agreement from the supplying university, providing that the DNA will not be used for commercial profit or gain. The sequence provided on this registry page has been provided with express permission from Clontech.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 352
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 352
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 352
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 352
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/rfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
color | Red |
direction | forward |
emission | 610 nm |
excitation | 587 nm |
n/a | DsRed derivative |
protein | mCherry |