Coding
Part:BBa_K5280129
Designed by: Hua XU Group: iGEM24_HKUST-GZ (2024-09-07)
LexRO
LexRO is a recombinant protein consisting of two parts: a DNA binding domain of LexA(408) repressor and a RsLOV light sensor domain. In darkness, its LOV domain dimerises and forms LexRO dimers, which can bind to the promoter and repress the expression of downstream genes. Under exposure to blue lights (450~465nm), the dimers dissociate, release the expression of downstream genes.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 205
Illegal PstI site found at 445
Illegal PstI site found at 514 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 205
Illegal PstI site found at 445
Illegal PstI site found at 514
Illegal NotI site found at 587 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 205
Illegal PstI site found at 445
Illegal PstI site found at 514 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 205
Illegal PstI site found at 445
Illegal PstI site found at 514 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |