Regulatory

Part:BBa_K180000:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-12)
Revision as of 10:28, 21 October 2009 by Rich (Talk | contribs) (→‎Design Notes)

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Hybrid promoter (trp & lac regulated -- tac pR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence here is preceeded by a coding region for lacIq; the function of this is to repress the promoter in it's natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state. When IPTG is no longer present in the system, the promoter will revert to it's non-induced state of 'OFF'.

Source

pMAL-pIII (New England Biolabs N8101S)

References

[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25