Reporter

Part:BBa_K5198012

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-30)
Revision as of 01:44, 5 September 2024 by Rk320 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


sfGFP (human codon optimized)

Human codon optimized sequence of superfolder Green Fluorescent Protein (sfGFP) [1]. The start codon is not included.

sfGFP is a mutated version of GFP (BBa_E0040) designed for stability and better folding. This variant of sfGFP can be used as a reporter of gene expression in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] J.-D. Pédelacq, S. Cabantous, T. Tran, T. C. Terwilliger, and G. S. Waldo, “Engineering and characterization of a superfolder green fluorescent protein,” Nat Biotechnol, vol. 24, no. 1, pp. 79–88, Jan. 2006, doi: 10.1038/nbt1172.

[edit]
Categories
Parameters
None