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Part:BBa_K5317001

Designed by: Jan Gelhoet   Group: iGEM24_Hannover   (2024-09-04)
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mRuby2

PknB is a eukaryote-like serine/threonine kinase in Staphylococcus aureus that consists of an N-terminal cytosolic kinase domain, a central transmembrane domain and three C-terminal extracellular PASTA domains.2 When this extracellular domain recognises beta-lactam compounds, it transmits the signal by autophosphorylating the N-terminal kinase domain, thereby activating downstream signalling cascades.3 In S. aureus, this mechanism normally enables early recognition and adaptation to antibiotic stress.4 In our project, we aim to introduce PknB into HEK cells, together with three proteins that can be phosphorylated by PknB. This targeted phosphorylation increases DNA binding activity 1 and is expected to induce the expression of a specific gene, which generates a fluorescent signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


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