Coding
creA

Part:BBa_K5108003:Design

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)
Revision as of 14:47, 28 August 2024 by Registry (Talk | contribs)

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Creatinase from Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 225
    Illegal BsaI site found at 1012


Design Notes

Codon optimized nucleotidic sequence from Pseudomonas putida adapted to Pseudomonas putida is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies (https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt). This step permits to lower complexity and minimize secondary structures.


Source

Uniprot P38488 (https://www.uniprot.org/uniprotkb/P38488/entry)

References