Composite

Part:BBa_K185002

Designed by: Alex Jiang   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-16)
Revision as of 07:13, 21 October 2009 by Kermit tao (Talk | contribs)

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Lon Protease+double terminater

The Lon protease is conneced by double terminator BBa_B0015.Take attention that this part contains a PstI restriction site. So if someone wants to use this part, it is better to cut it with SpeI in the suffix region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 619
    Illegal AgeI site found at 1795
    Illegal AgeI site found at 1879
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2110
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None