Part:BBa_K5384004

AOX 1 promoter

The Pichia Pastris alcohol oxidase 1 (AOX1) promoter variant is characterized by comprising at least one of the specified modifications in the wild-type Pikia Pastris AOX1 promoter of SEQ ID NO: 1. a) Nucleotides 94-110 (-847-831), Nucleotides 141-160 (-800--781), Nucleotides 312-330 (-629-6-111), Nucleotides 355-380 (-586-561), Nucleotides 501-521 (-440-420); inside nucleotides 640-658 (-301--283), nucleotides 674-693 (-267--248) and nucleotides 1-840 (-940--100). Integration of the Cat8 transcription factor binding site (TFBS) at any position, in particular the integration of the "TTCCGTTCGTCCGA" gene sequence or other gene sequence showing at least 80％ similarity to this sequence; b) Nucleotides 1-840 (-). Integration of Aca1 or Aca2 TFBS at any position between 940--100), particularly integration of the "GCCATTTGTAGACGTCAACCC" nucleotide sequence or other gene sequence showing at least 80％ similarity to this sequence; c) Nucleotide 94 Within ~ 693 (-847 to -248), the mutation specified by SEQ ID NO: 2 as well as the mutation selected from the group consisting of combinations thereof are included, and the AOX1 promoter variant further comprises the wild-type AOX1 promoter described above. When compared to, it is characterized by its increased strength and guiding ability.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]