Composite

Part:BBa_K5198102

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-23)
Revision as of 17:49, 23 August 2024 by Registry (Talk | contribs)

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IFN_SEAP

Secreted Embryonic Alkaline Phosphatase with IFN tag. This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 589
    Illegal NgoMIV site found at 1558
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 919
    Illegal BsaI.rc site found at 1408


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Categories
Parameters
None