Tag

Part:BBa_K5198011:Design

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-23)
Revision as of 15:40, 23 August 2024 by Rk320 (Talk | contribs) (→‎References)

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CTR tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence needs to be added to N-terminus of the protein cargo to secrete properly.


Source

The amino acid sequence was reverse transcribed and codon optimized for expression in mammalian cells.

References

“Commonly Used Leader Peptide Sequences for Mammalian Cells Expression.” NovoPro 21 Apr. 2018, https://www.novoprolabs.com/support/articles/commonly-used-leader-peptide-sequences-for-efficient-secretion-of-a-recombinant-protein-expressed-in-mammalian-cells-201804211337.html.