Intermediate

Part:BBa_K218003:Design

Designed by: Jeremy Kubik   Group: iGEM09_Calgary   (2009-10-19)
Revision as of 06:03, 21 October 2009 by Jfkubik (Talk | contribs) (→‎Design Notes)

LuxOU from Vibrio harveyi followed by terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 854
    Illegal SapI.rc site found at 816


Design Notes

LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The internal RBS was also kept, and although there is an internal terminator as well, we have constructed the strong terminator B0015 after this coding sequence in order to provide further insulation of the circuit.

Source

blah

References