Coding

Part:BBa_K5198000:Design

Designed by: Duke iGEM   Group: iGEM24_Duke   (2024-08-20)
Revision as of 08:09, 21 August 2024 by Rk320 (Talk | contribs) (→‎Design Notes)


CD19ecto


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 225
    Illegal SapI.rc site found at 61


Design Notes

The ectodomain sequence was determined based on the annotation in UniProt entry.

Source

The sequence was created by reverse transcription of human CD19 protein (UniProt: P15391). The ectodomain was codon optimized for expression in human cells.

References