Coding
Part:BBa_K5387000:Design
Designed by: iGEM24_Linkoping Group: iGEM24_Linkoping (2024-08-20)
Revision as of 12:49, 20 August 2024 by Feliciawedlund (Talk | contribs)
Design Notes
The sequence has been optimized for expression in E. coli.
Primers for cloning into pET28a(+) between restriction sites NdeI and EcoRI:
- fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
- rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'
Source
This gene is expressed in humans KÄLLA