Coding

Part:BBa_K5387000:Design

Designed by: iGEM24_Linkoping   Group: iGEM24_Linkoping   (2024-08-20)
Revision as of 08:50, 20 August 2024 by Feliciawedlund (Talk | contribs) (References=)

Design Notes

The sequence has been optimized for expression in E. coli.

Primers for cloning into pET28a(+) between restriction sites NdeI and EcoRI:

fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'

Source

This gene is expressed in humans KÄLLA


References