Coding
Part:BBa_K5387001:Design
Designed by: iGEM24_Linkoping Group: iGEM24_Linkoping (2024-08-20)
Revision as of 08:14, 20 August 2024 by Feliciawedlund (Talk | contribs)
ALOXE3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
Illegal BglII site found at 1541
Illegal BglII site found at 1646 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1435
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1920
Description
ALOXE3 encodes for the protein Epidermis-type lipoxygenase-3 (eLOX-3), an enzyme which oxidizes fatty acids to create fatty acids hydroperoxides [1]. The gene codes for one of the enzymes used for iGEM24_Linkoping substitution therapy for Ichthyosis.
Design Notes
The sequence has been optimized for expression in E. coli.
The sequence contains the amino acid sequence "QPPP", which is very difficult for the ribosomes in E. coli to translate. We did not do it, but for future projects we would recommend altering this part of the sequence, either by mutation or when ordering the sequence.
Source
This gene is expressed in humans.