Coding
Part:BBa_K5387001:Design
Designed by: iGEM24_Linkoping Group: iGEM24_Linkoping (2024-08-20)
Revision as of 07:51, 20 August 2024 by Feliciawedlund (Talk | contribs)
ALOXE3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
Illegal BglII site found at 1541
Illegal BglII site found at 1646 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1435
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1920
Design Notes
The sequence has been optimized for expression in E. coli.
The sequence contains the amino acid sequence "QPPP", which is very difficult for the ribosomes in E. coli to translate. We did not do it, but for future projects we would recommend altering this part of the sequence, either by mutation or when ordering the sequence.
Source
This gene is expressed in humans.