Coding

Part:BBa_K5387001:Design

Designed by: iGEM24_Linkoping   Group: iGEM24_Linkoping   (2024-08-20)
Revision as of 07:46, 20 August 2024 by Registry (Talk | contribs)

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ALOXE3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
    Illegal BglII site found at 1541
    Illegal BglII site found at 1646
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1435
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1920


Design Notes

The sequence has been optimized for expression in E. coli.

The sequence contains the amino acid sequence "QPPP", which is very difficult for the ribosomes in E. coli to translate. We did not do it, but for future projects we would recommend altering this part of the sequence, either by mutation or when ordering the sequence.


Source

This gene is expressed in humans.

References