Regulatory

Part:BBa_K5226018

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-08-18)
Revision as of 08:14, 18 August 2024 by Registry (Talk | contribs)

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porin278

Lac Operator sequence that is bound by LacI repressor. When attached downstream of a promoter this will inhibit transcription of any genes downstream. Therefore, this part can be used to create a repressed promoter belonging to any class. In order to reduce the leakage level of the Mmp1 promoter, one more lacO was introduced immediately after the transcription start site (+1) of PT7-like (producing PT7-like-LacO).The Mmp1 inducible promoter currently used by our team has undergone various optimizations as described above.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None