RBS

Part:BBa_K5226024

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-08-18)
Revision as of 05:50, 18 August 2024 by Admin (Talk | contribs)


RBS for Vib-gcvT

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).

Usage and Biology

This part is a native ribosomal binding site of Vib-gcvT, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of aminomethyltransferase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None