Coding
Part:BBa_K5043006
Designed by: Richard Meuten Group: iGEM24_TUDarmstadt (2024-08-17)
pdoA2 from Mycobacterium sp. strain 6PY1
Encodes Phenanthrene ring-hydroxylating oxygenase, α subunit. Presumably catalyzes as complex with pdoB2, phtAc and phtAd oxidation of Phenanthrene-4-carboxylate to cis-phenanthrene-3,4-dihydrodiol-4-carboxylate.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1285
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1285
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1285
Illegal XhoI site found at 448 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1285
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1285
Illegal NgoMIV site found at 43
Illegal NgoMIV site found at 723 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |