Coding

Part:BBa_K5043006

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-08-17)
Revision as of 18:06, 17 August 2024 by Registry (Talk | contribs)

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pdoA2 from Mycobacterium sp. strain 6PY1

Encodes Phenanthrene ring-hydroxylating oxygenase, α subunit. Presumably catalyzes as complex with pdoB2, phtAc and phtAd oxidation of Phenanthrene-4-carboxylate to cis-phenanthrene-3,4-dihydrodiol-4-carboxylate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1285
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1285
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1285
    Illegal XhoI site found at 448
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1285
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1285
    Illegal NgoMIV site found at 43
    Illegal NgoMIV site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None