Part:BBa_K5043002

nidB from M. vanbaalenii Pyr-1

nidB forms together with nidA, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyses the oxidation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.

This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrading bacteria. It was also reported that nidAB is able to oxidize other high molecular weight polycyclic aromatic hydrocarbons. [1]

[1] O. Kweon, S.-J. Kim, J. P. Freeman, J. Song, S. Baek, and C. E. Cerniglia, "Substrate specificity and structural characteristics of the novel Rieske nonheme iron aromatic ring-hydroxylating oxygenases NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1," mBio, vol. 1, no. 2, 2010, doi: 10.1128/mBio.00135-10

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 395
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 395
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 395
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 395
  
• 1000
  COMPATIBLE WITH RFC[1000]