Part:BBa_K5043001

nidA from M. vanbaalenii Pyr-1

nidA forms together with nidB, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyses the oxidation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.

This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrading bacteria. It was also reported that nidAB is able to oxidize other high molecular weight polycyclic aromatic hydrocarbons. [1]

[1] O. Kweon, S.-J. Kim, J. P. Freeman, J. Song, S. Baek, and C. E. Cerniglia, "Substrate specificity and structural characteristics of the novel Rieske nonheme iron aromatic ring-hydroxylating oxygenases NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1," mBio, vol. 1, no. 2, 2010, doi: 10.1128/mBio.00135-10

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 760
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 760
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 752
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 760
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 760
  Illegal NgoMIV site found at 96
  Illegal NgoMIV site found at 399
  
• 1000
  COMPATIBLE WITH RFC[1000]