Coding

Part:BBa_K5124000

Designed by: Louise Brown   Group: iGEM24_Exeter   (2024-08-15)
Revision as of 11:15, 15 August 2024 by Registry (Talk | contribs)

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LbCas12a codon opt

CRISPR-associated protein from Lachnospiraceae bacterium ND2006 (Zetsche et al., 2015) E coli optimised Moreno-Mateos et al 2017


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1461
    Illegal BglII site found at 2198
    Illegal BglII site found at 3210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3112
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None