Regulatory
P_PTI1
Part:BBa_K5088000
Designed by: Dascha Khalfine Group: iGEM24_Marburg (2024-07-30)
Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz
Contents
Background
Motivation
Use Cases
Lack of Endogenous Regulatory Parts
Political Context
Plant Synthetic Biology
Gene Structure in Plants
Cis-Regulatory Elements
Design and Characterization
Identification & Design Strategy
Genetic Context of the Part
Measurement
Dual Fluorescence Reporter Assay
Choice of Reporter Genes
Transient Transformation
Leaf Infiltration
Protoplast
Measurement Setup
Results
The Dandelion Toolbox
Overview
Part Identifier | Part Type | Nickname | Part Description |
---|---|---|---|
BBa_K5088000 | Promoter + 5'UTR | P_PTI1 | Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088001 | Promoter + 5'UTR | P_RPL28 | Large subunit ribosomal protein L28e - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088002 | Promoter + 5'UTR | P_GSK3B | Glycogen synthase kinase 3 - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088003 | Promoter + 5'UTR | P_MGRN1 | E3 ubiquitin-protein ligase - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088004 | Promoter + 5'UTR | P_betB | Betaine-aldehyde dehydrogenase - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088005 | Promoter + 5'UTR | P_pgm | Phosphoglucomutase - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088006 | Promoter + 5'UTR | P_FKBP4_5 | FK506-binding protein 4/5 - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088007 | Promoter + 5'UTR | P_CLTC | Clathrin - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088008 | Promoter + 5'UTR | P_RPL31 | Large subunit ribosomal protein L31e - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088009 | Promoter + 5'UTR | P_CUL1 | Cullin - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088010 | Promoter + 5'UTR | P_VPS4 | Vacuolar protein-sorting-associated protein 4 - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088011 | Promoter + 5'UTR | P_EIF2S3 | Translation initiation factor 2 subunit 3 - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088012 | Promoter + 5'UTR | P_Tubulin | Tubulin - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088013 | Promoter + 5'UTR | P_EIF5A | Translation initiation factor 5A - Promoter+5'UTR from T. kok-saghyz |
BBa_K5088050 | Inducible Promoter + 5'UTR | P_HSP12.6 | HSP12.6 - Heat inducible promoter+5'UTR from T. koksaghyz |
BBa_K5088051 | Inducible Promoter + 5'UTR | P_HSP23.5 | HSP23.5 - Heat inducible promoter+5'UTR from T. koksaghyz |
BBa_K5088102 | 3'UTR | T_PTI1 | Protein tyrosine kinase - 3'UTR from T. kok-saghyz |
BBa_K5088103 | 3'UTR | T_RPL28 | Large subunit ribosomal protein L28e - 3'UTR from T. kok-saghyz |
BBa_K5088104 | 3'UTR | T_EPS15 | Epidermal growth factor receptor substrate 15 - 3'UTR from T. kok-saghyz |
BBa_K5088105 | 3'UTR | T_GSK3B | Glycogen synthase kinase 3 - 3'UTR from T. kok-saghyz |
BBa_K5088106 | 3'UTR | T_MGRN1 | E3 ubiquitin-protein ligase - 3'UTR from T. kok-saghyz |
BBa_K5088107 | 3'UTR | T_RPL35A | Large subunit ribosomal protein L35Ae - 3'UTR from T. kok-saghyz |
BBa_K5088108 | 3'UTR | T_betB | Betaine-aldehyde dehydrogenase - 3'UTR from T. kok-saghyz |
BBa_K5088109 | 3'UTR | T_pgm | Phosphoglucomutase - 3'UTR from T. kok-saghyz |
BBa_K5088110 | 3'UTR | T_ATP-synt | ATPase subunit gamma - 3'UTR from T. kok-saghyz |
BBa_K5088111 | 3'UTR | T_EIF3B | Translation initiation factor 3 subunit B - 3'UTR from T. kok-saghyz |
BBa_K5088112 | 3'UTR | T_RPL31 | Large subunit ribosomal protein L31e - 3'UTR from T. kok-saghyz |
BBa_K5088113 | 3'UTR | T_TM9SF2_4 | Transmembrane 9 superfamily member 2/4 - 3'UTR from T. kok-saghyz |
BBa_K5088114 | 3'UTR | T_CUL1 | Cullin - 3'UTR from T. kok-saghyz |
BBa_K5088115 | 3'UTR | T_PSMB6 | 20S proteasome subunit beta 1 - 3'UTR from T. kok-saghyz |
BBa_K5088116 | 3'UTR | T_RPSA | Small subunit ribosomal protein SAe - 3'UTR from T. kok-saghyz |
BBa_K5088117 | 3'UTR | T_VPS4 | Vacuolar protein-sorting-associated protein 4 - 3'UTR from T. kok-saghyz |
BBa_K5088118 | 3'UTR | T_EIF2S3 | Translation initiation factor 2 subunit 3 - 3'UTR from T. kok-saghyz |
Dandelion Handbook
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1262
Illegal XbaI site found at 184 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1262
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1262
Illegal BglII site found at 409
Illegal XhoI site found at 436 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1262
Illegal XbaI site found at 184 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1262
Illegal XbaI site found at 184
Illegal AgeI site found at 1761 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/plants/other
origin | Taraxacum kok-saghyz |