Regulatory
P_PTI1

Part:BBa_K5088000

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-07-30)
Revision as of 11:20, 11 August 2024 by Daschka (Talk | contribs) (→‎The Dandelion Toolbox)


Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz


Background

Motivation

Use Cases

Lack of Endogenous Regulatory Parts

Political Context

Plant Synthetic Biology

Gene Structure in Plants

Cis-Regulatory Elements

Design and Characterization

Identification & Design Strategy

Genetic Context of the Part

Figure X: Lorem ipsum dolor sit amen

Measurement

Dual Fluorescence Reporter Assay

Choice of Reporter Genes

Transient Transformation

Leaf Infiltration

Protoplast

Measurement Setup

Results

The Dandelion Toolbox

Overview

Part Identifier Part Type Nickname Part Description
BBa_K5088000 Promoter + 5’UTR P_PTI Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz
BBa_K5088001 Promoter + 5’UTR P_RPL28 Large subunit ribosomal protein L28e - Promoter+5'UTR from T. kok-saghyz
BBa_K5088002 Promoter + 5’UTR P_GSK3B Glycogen synthase kinase 3 - Promoter+5'UTR from T. kok-saghyz

Dandelion Handbook

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1262
    Illegal BglII site found at 409
    Illegal XhoI site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
    Illegal AgeI site found at 1761
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/plants/other
Parameters
originTaraxacum kok-saghyz