DNA
PopdH

Part:BBa_K5291021:Design

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-08-11)
Revision as of 02:33, 11 August 2024 by Kortybones (Talk | contribs) (→‎References)


PopdH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We choose this gene because the opdH-tctCBA-tctDE manipulator system natively exists in Pseudomonas aeruginosa, which means that we do not need to introduce more regulatory genes into the engineered bacteria. We can just put the PopdH promoter into Pseudomonas aeruginosa and the tctD in it will naturally come into effect.


Source

Pseudomonas aeruginosa

References

[1]Bielecki, P., et al., Cross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa. Nucleic Acids Res, 2015. 43(13): p. 6413-25. [2]Tamber, S., et al., Characterization of OpdH, a Pseudomonas aeruginosa porin involved in the uptake of tricarboxylates. J Bacteriol, 2007. 189(3): p. 929-39. [3]Brocker, M., et al., Citrate utilization by Corynebacterium glutamicum is controlled by the CitAB two-component system through positive regulation of the citrate transport genes citH and tctCBA. J Bacteriol, 2009. 191(12): p. 3869-80. [4]Underhill, S. and M.T. Cabeen, Redundancy in Citrate and cis-Aconitate Transport in Pseudomonas aeruginosa. J Bacteriol, 2022. 204(12): p. e0028422.